Measurement
lacITst

Part:BBa_S03469

Designed by: ETH Zurich iGEM Team 2005   Group: iGEM05_ETHZ   (2006-05-02)


Lambda-lacI input system TESTER (IPTG controlled lacI+pL efficiency)

Constitutive (tetR) lacI-production (lacI+LVA) to block promoter (lacI+pL) in ground state of a 2-state system. lacI+pL can be activated, e.g. over IPTG, thus leading to the production of CFP (yellow fluorescence). This device allows to estimate repressed (lacI) activity/full activity under ITPG absence/presence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None