Part:BBa_R2002

Promoter, Zif23 regulated, test: between and after

Promoter with standard -10 and -35 sites. A symmetric Zif23 dimer target site is between the -10 and -35 sites, and a symmetric Zif23 dimer target site is after the -10 site.

Usage and Biology

See [Wolfe00] regarding Zif23.

This promoter successfully drives transcription of GFP when placed upstream of BBa_E0840. It is a strong promoter. When placed upstream of BBa_E0240, fluorescence levels are comparable to background.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]