Part:BBa_P1014

gentamicin resistance cassette aph(2)

Novel gentamicin resistance gene. Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray. This, in turn was derived from Genbank AF016483. See pmid=16391062, pmid=9593155

Conveys resistance to genamicin at up to 12 ug/ml. It conveys low levels of resistance to kanamycin, at 1.5 ug/ml. E. coli NEB10B cells are sensitive to gentamicin at less than 0.3 ug/ml. Selection for this cassette carried out at 4 ug/ml will allow growth in Gentamicin, but inhibit other growth. With standard kanamycin concentrations of 35 ug/ml, this cassette will not convey Kanamycin resistance.

Gentamicin is an important antibiotic for use in human disease. Its use should be carefully considered and alternatives sought when used in a context promoting easy horizontal transfer of the cassette to potential human pathogens, for example in mobilizable plasmids or in transposable elements (insertions sequences).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]