Part:BBa_P1003

kanamycin resistance cassette

Kanamycin resistance cassette including promoter and coding sequence. It lacks a terminator.

Usage and Biology

• Used for modular BioBrick vector construction using BioBrick base vector BBa_I51020.

MiamiU_OH Contribution

This part was optimized for expression and function in the photosynthetic chassis Synechococcus elongatus PCC 7942. The promoter can be found at BBa_K4047020 and the coding sequence can be found at BBa_K4047019.

References

Engineering BioBrick vectors from BioBrick parts
Journal of Biological Engineering, 2008 Apr 14;2:5
Reshma Shetty, Drew Endy, Tom Knight
[http://www.jbioleng.org/content/2/1/5 URL]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=169656101 GenBank EU496093]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]