Part:BBa_P0412

PoPS -> LacI [S0150]

Measurement

Design
NOT logic gate is a common and useful part in logic gene circuit. This year, igem16-JNFLS-China team construct a device to identify the proper dosage of inducer to achieve a better inductive effect. In the device, we considered 5 strength constitutive promoters and used GFP to report the inductive effect (fig1). The induced degree is up to the response effect in a steady state and the speed of the effect depends on the dynamic process, these 2 points are very essential in logic gate. Therefore we test the dynamic process of different quantity of the repressor and different concentration of inducer. After that we did the steady-state analysis and the dynamic analysis. In the end we wish that it can be a reference method for the function of logic gate circuit for the future team to describe their elements' function.

Figure 1: Construct design.

Results
The response condition is similar to what we test in TetR-Ptet (BBa_K173005)gene circuit There are still some problems, for instance in fig.2, the response condition became very fast as we used a weak promoter. This issue occurs because of the choosing of promoters and the concentration level of the inducer. It is uncertain for every single element. Therefore, how to maintain the comparability of the parameter as while as acquiring a significant result is on the top of our agenda. Nevertheless, does our promoters as stable as we thought? We cannot make sure and we have figured that during testing. It is the reason of the uncertainty of most elements. This also stimulate the synthetic biology field to set up a qualify standard to describing and submitting.

Figure 2.

While quantitative biology is developing step by step, some special model can get involve. At that time we will be able to use more qualify and more elegant language to describe the characteristics of elements and the foundation of synthetic biology will be more mature. We are looking forward that day!

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1165
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]