PoPS -> TetR [S0163]
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Tianjin's 2021 characterization
New information of TetR operon leakage
Group: Tianjin 2021
Author: Ruiqi Liu, Lingwei Ding
Summary: we added information about TetR operon leakage.
In our project, we want to use TetR operon to control the expression of mcherry gene. Thus, we want to test whether TetR operon can strictly regulate the expression of gene or not. We constructed a carotene plasmid with TetR operon, and transformed it into Saccharomyces cerevisiae. If the carotene gene controlled by TetR operon leaks, the yeasts will turn orange.
We cultured the yeasts after transforming the carotene plasmid. We were surprised to find that, before using Atc inducer, a large proportion of yeasts was orange. Our experimental results show that the tetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to control. Therefore, the other iGEMers should aware that TetR operon is not a good choice if you want to strictly control the expression of structure gene.