Coding

Part:BBa_M50054

Designed by: Lucy Maynard, Doris Mai and Amy Weissenbach   Group: Stanford BIOE44 - S11   (2016-12-11)


PETase double mutant I208V and R90A

This is a mutant of PETase that combines two mutations designed by the 2016 iGEM Tianjin team. Wild-type PETase shows the ability to hydrolyze Polyethylene terephthalate (PET), commonly used for plastics. The two mutations are the 208th amino acid (changed from isoleucine(I) to valine(V)) and the 90th amino acid (changed from arginine (R) to alanine (A)), and each individual mutant has been showed by the Tianjin team to improve the catalytic activity of PET. This sequence combines both two mutations and is added 6 his tag at the end. The sequence is optimized for Escherichia coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 633
    Illegal PstI site found at 864
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 633
    Illegal PstI site found at 864
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 633
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 633
    Illegal PstI site found at 864
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 633
    Illegal PstI site found at 864
    Illegal NgoMIV site found at 220
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 388


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Categories
Parameters
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