Part:BBa_M50053
FRET-based glucose sensor using a glucose binding protein, mRuby3 and cometGFP
This is a device that uses a stable glucose binding protein (GBP) flanked with two compatible fluorescent proteins to indicate glucose levels with a fluorescent resonance energy transfer (FRET) based actuator. The device input is glucose-binding and the device output is fluorescent spectra of the two flurophore. FRET was achieved using mRuby3 as the acceptor fluorophore and cometGFP as the donor fluorophore. Upon glucose binding, this device will undergo a conformational change such that the cometGFP protein in an excited state would transfer energy to the acceptor mRuby3 to be emitted. Quantifying this excitation/emission pair would reveal the extent to which FRET and thus glucose binding that had occurred, relative to the open, unbounded state.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 77
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