DNA

Part:BBa_M50011

Designed by: Tracy Lord and Michael Eseigbe (Team ME-TL)   Group: Stanford BIOE44 - S11   (2016-10-27)


This is an E. coli specific sensor for hydrogen peroxide.

Based on the HyPer gene, this is an E. coli specific genetic design that allows E. coli to detect the presence of hydrogen peroxide.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 844
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25
    Illegal SapI.rc site found at 709


Usage and Biology

Regarding the biology field, having bacteria that is able to sense the presence of hydrogen peroxide is valuable. Hydrogen peroxide in high concentrations can be deadly to bacteria and cells in general, and it is important for biologists to know the amount of hydrogen peroxide that may be building up as the cells grow. Having bacteria with the ability to determine the presence of specific concentrations of hydrogen peroxide would allow the scientists to detect potentially toxic levels of hydrogen peroxide in their experiments and work.



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