Composite

Part:BBa_M45902

Designed by: Chad Lawrence Nielsen   Group: UtahState_BE5930_-_S14   (2015-04-10)


Botulism Toxin Type C Detector

This part is designed to detect the presence of botulism toxin type C. It is composed of a ribosome binding site, a fluorescent protein (SBFP2), a cleavage site that the toxin attacks (Syntaxin-1A), and a 10x his-tag. Ideally, this should be able to be purified after production using a nickel or copper column, eluted, and then mixed with the sample. If the toxin is in solution, it will cleave the Syntaxin-1A sequence, separating the fluorescent protein from the his-tag. This means that if the solution is run through a column again, the fluorescent protein should come out during the flow throughs rather than the elutions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1079
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 36


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Categories
Parameters
None