T7

Part:BBa_M45802

Designed by: Thomas Overbeck   Group: UtahState_BE5930_-_S14   (2015-04-15)


Mutated Tuc2009 bidirectional promoter for decreased CI sensitivity

A modified bi-directional promoter from lactococcal bacteriophage Tuc2009 containing a bp mutation for decreased binding affinity of the CI repressor protein to the operator OL-CI at high CI concentrations. Allowing for continued up-regulated transcription from the leftward promoter (PL). See BBa_M45801 for detailed description of bi-directional promoter and intergenic switch region regulation with CI and Cro repressor proteins.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None