T7

Part:BBa_M45801

Designed by: Thomas Overbeck   Group: UtahState_BE5930_-_S14   (2015-04-15)


Bi-directional lactococcal bacteriophage Tuc2009 promoter

Bi-directional promoter containing a divergent rightward (PR) and leftward (PL) promoter from Tuc2009 bacteriophage with an intergenic switch region negatively regulated by CI and Cro repressor proteins, respectively. The intergenic switch region contains four operator regions designated OL-Cro, OR-Cro, OL-CI, and OR-CI. Cro has high affinity binding to OL-Cro, resulting in repression of PL and up-regulation of PR. At high concentrations Cro will bind to OR-Cro and repress PR. CI has high affinity binding to OR-CI, resulting in repression of PR and up-regulation of PL. At high concentrations CI will bind to OL-CI and repress PL.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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