Composite

Part:BBa_M45505

Designed by: Dallin V. Christensen   Group: UtahState_BE5930_-_S14   (2015-04-23)


pRM promoter with phiR73 delta that is used to activate the promoter PF

Lambda Prm promoter was modified to be activated but not repressed by the lambda repressor Cl. This gives the promoter the ability to be sense when a phage has infected the cell. The delta activator, designed by Cambridge 2007, was taken from phiR73 phage and acts on a class of inducible promoters (parts I746360 to I746365). The part sequence does already contain a ribosome binding site (B0034). A double stop codon B0015 is also added to the back of the part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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