Composite

Part:BBa_M45504

Designed by: Dallin V. Christensen   Group: UtahState_BE5930_-_S14   (2015-04-23)


PF promoter with cell lysis device

This is the cell lysis device designed by UC Berkeley in 2008 with the PF promoter that is activated by the protein phiR73. The lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin. Antiholin has its own constitutive promoter to prevent the formation of holin multimers from basal expression. When the device is off, higher expression of antiholin compared to holin is required for better stability of the device.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1469
    Illegal NheI site found at 1492
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 18
    Illegal AgeI site found at 1074
    Illegal AgeI site found at 1144
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1725


[edit]
Categories
Parameters
None