Coding

Part:BBa_M45157

Designed by: Cynthia Hanson   Group: UtahState_BE5930_-_S14   (2016-04-20)


Amino acid sequence to produce MPT64

This is an amino acid sequence taken from Mycobacterium tuberculosis to produce the protein MPT64. The amino acid sequence has been changed to be E.coli-favorable, and restriction enzyme sites for EcoRI, PstI, SpeI, and XbaI have been removed. This sequence can be used as a means to produce protein MPT64 without culturing M. tuberculosis, and therefore can be used as a means to test for the presence of M. tuberculosis without the risk of the bacteria being in the lab.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 456
    Illegal BamHI site found at 546
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 538
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 519


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Categories
Parameters
None