Composite

Part:BBa_M45125

Designed by: Sherissa Ward   Group: UtahState_BE5930_-_S14   (2014-04-21)

F-Plasmid OriT with Autolysin for Cell Death Induced Conjugation

OriT is the nic region on the F Plasmid that allows for conjugation in the presence of transfer genes encoded in the Tra gene region. This OriT gene is followed by an autolysin N-acetylmuramoyl-L-alanine amidase. This codes for an Autolysin that will cause cell death to the cell upon conjugation. This composite part is meant to be incorporated into a plasmid with the TraJ genes knocked out with an antibiotic resistance gene. This will further prevent conjugation by the bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 234
    Illegal NheI site found at 280
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 381
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None