Composite

Part:BBa_M45101

Designed by: Mitchell Bullough   Group: UtahState_BE5930_-_S14   (2014-04-14)


Composite biobrick intended to induce biofilm formation in the presence of lactose. Made from parts

Using a LacZ operon (with RBS in sequence), curli genes are expressed and ultimately induce biofilm formation. The intent of this biobrick is to turn on biofilm formation in the presence of lactose. This allows bacteria to be cultured normally and then seeded as a biofilm when lactose is added. Biofilms have a specialized metabolism and are more resilient to toxins, improving the ability for bioremediation of toxins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 745
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None