Composite

Part:BBa_M41006

Designed by: Hao Xing   Group: MIT BE 20.20-S13   (2013-05-12)


Hydrocarbon Sensor

This is a sequence for hydrocarbon sensor. The input for this sensor is n-alkane chain and output is LacI. It has a sequence which encodes membrane protein (AlkL) and chemotaxis protein (AlkN) responsible for hydrocarbon sensing. This sequence does not code for any major alkane degrading enzymes except AlkL and AlkN.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4274
    Illegal SpeI site found at 4643
    Illegal PstI site found at 2764
    Illegal PstI site found at 3903
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1679
    Illegal SpeI site found at 4643
    Illegal PstI site found at 2764
    Illegal PstI site found at 3903
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4516
    Illegal BglII site found at 6633
    Illegal XhoI site found at 392
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4274
    Illegal SpeI site found at 4643
    Illegal PstI site found at 2764
    Illegal PstI site found at 3903
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4274
    Illegal SpeI site found at 4643
    Illegal PstI site found at 2764
    Illegal PstI site found at 3903
    Illegal NgoMIV site found at 3269
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3431
    Illegal BsaI.rc site found at 2902
    Illegal BsaI.rc site found at 3204


[edit]
Categories
Parameters
None