Coding

Part:BBa_M41004

Designed by: Hao Xing   Group: MIT BE 20.20-S13   (2013-05-12)


AlkS coding sequence in Pseudomonas putida

This gene encodes a transcription factor AlkS, which activates alkane degradation operon. AlkS self-regulates its expression. In the presence of alkanes, AlkS forms AlkS-hydrocarbon (AlkS-HC) complex which downregulates pAlkS1 and upregulates pAlkS2. AlkS-HC complex activates pAlkB which is responsible for expression of alkane degradation operon.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1731
    Illegal SpeI site found at 2100
    Illegal PstI site found at 221
    Illegal PstI site found at 1360
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2100
    Illegal PstI site found at 221
    Illegal PstI site found at 1360
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1973
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1731
    Illegal SpeI site found at 2100
    Illegal PstI site found at 221
    Illegal PstI site found at 1360
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1731
    Illegal SpeI site found at 2100
    Illegal PstI site found at 221
    Illegal PstI site found at 1360
    Illegal NgoMIV site found at 726
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 888
    Illegal BsaI.rc site found at 359
    Illegal BsaI.rc site found at 661


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