Coding
Part:BBa_M41004
Designed by: Hao Xing Group: MIT BE 20.20-S13 (2013-05-12)
AlkS coding sequence in Pseudomonas putida
This gene encodes a transcription factor AlkS, which activates alkane degradation operon. AlkS self-regulates its expression. In the presence of alkanes, AlkS forms AlkS-hydrocarbon (AlkS-HC) complex which downregulates pAlkS1 and upregulates pAlkS2. AlkS-HC complex activates pAlkB which is responsible for expression of alkane degradation operon.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1731
Illegal SpeI site found at 2100
Illegal PstI site found at 221
Illegal PstI site found at 1360 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 2100
Illegal PstI site found at 221
Illegal PstI site found at 1360 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1973
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1731
Illegal SpeI site found at 2100
Illegal PstI site found at 221
Illegal PstI site found at 1360 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1731
Illegal SpeI site found at 2100
Illegal PstI site found at 221
Illegal PstI site found at 1360
Illegal NgoMIV site found at 726 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 888
Illegal BsaI.rc site found at 359
Illegal BsaI.rc site found at 661
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Categories
Parameters
None |