Regulatory

Part:BBa_M41002

Designed by: Hao Xing   Group: MIT BE 20.20-S13   (2013-05-12)


pAlkS1 promoter in Pseudomonas putida

It is a first promoter of AlkS gene which is recognized by σS-RNA polymerase. It is downregulated by AlkS protein in the absence of alkanes, keeping the level of AlkS constant in the cell. When there are alkanes present in the cell, AlkS-hydrocarbon complex represses pAlkS1 and activates second promoter, pAlkS2, to produce more AlkS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None