Regulatory

Part:BBa_M39413

Designed by: Zach Banks   Group: MIT BE 20.20-S12   (2012-05-15)

flipable pCat promoter regulated by fimE recombinase

pCat promoter (BBa_I14033) flanked by regions (BBa_K137010, BBa_K137008) recognized by fimE recombinase (BBa_K137007).

pCat is a constitutive promoter, which enables this device to be used as a switch to constitutively express two different regions. By default, the downstream sense strand is promoted as usual. When flipped, the upstream antisense strand is promoted.

Additionally, upstream of the promoter is a terminating sequence BBa_B0015. This sequence is to prevent "backwards" transcription before the promoter is flipped. It is between the recombinant regions, causing it to flip with the promoter.

pCat can be replaced with another promoter for additional regulation if needed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None