Device

Part:BBa_M39412

Designed by: Zach Banks   Group: MIT BE 20.20-S12   (2012-05-15)


DNAseI generator inhibited by recombinant pCat promoter

DNAseI generating module that is inhibited by (lack of) recombination. Initially, the promoter is set to produce actin with a fast degradation tag, which inhibits DNAseI.

When fimE is expressed elsewhere in the cell, the promoter is flipped to stop production of actin. Instead, DNAseI is expressed, along with ECP32 which degrades actin. Additionally, tetR is produced to be used in other regulatory mechanisms.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None