Part:BBa_M39123
Glucose->Insulin
This device is made from several different parts taken from E. coli and Yeast. Our goal in creating this device was to be able to measure glucose levels and have the cells then produce an appropriate amount of insulin in response. To do this, we used the following parts: CAP binding site from the Lac Operon (taken from E. coli.), the Promoter found in the Lac Operon, a yeast ribosome binding site, the cDNA for human insulin, and a yeast terminator. We plan to use this device in yeast. As glucose is taken in, levels of cAMP increase. cAMP binds to the CAP binding site, facilitating the binding of RNA polymerase to the promoter and allowing transcription of the insulin gene. As glucose levels rise, so does that of cAMP, increasing the amount of insulin produced. Once glucose levels lower, cAMP lowers, and there is less insulin produced.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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