Device

Part:BBa_M39023

Designed by: Edgar Aranda-Michel   Group: MIT BE 20.20-S12   (2012-05-14)


AND gate structure for regulation of E.coli in CF mucus

This device will have the cells live in the presence of LasL and Sialic acid

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1176
    Illegal XbaI site found at 44
    Illegal XbaI site found at 2568
    Illegal XbaI site found at 3524
    Illegal XbaI site found at 4276
    Illegal XbaI site found at 5350
    Illegal XbaI site found at 6446
    Illegal XbaI site found at 7369
    Illegal PstI site found at 1216
    Illegal PstI site found at 5762
    Illegal PstI site found at 6537
    Illegal PstI site found at 6945
    Illegal PstI site found at 7919
    Illegal PstI site found at 10025
    Illegal PstI site found at 10558
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1176
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 6409
    Illegal NheI site found at 6432
    Illegal PstI site found at 1216
    Illegal PstI site found at 5762
    Illegal PstI site found at 6537
    Illegal PstI site found at 6945
    Illegal PstI site found at 7919
    Illegal PstI site found at 10025
    Illegal PstI site found at 10558
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1176
    Illegal XhoI site found at 5999
    Illegal XhoI site found at 8246
    Illegal XhoI site found at 8255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1176
    Illegal XbaI site found at 44
    Illegal XbaI site found at 2568
    Illegal XbaI site found at 3524
    Illegal XbaI site found at 4276
    Illegal XbaI site found at 5350
    Illegal XbaI site found at 6446
    Illegal XbaI site found at 7369
    Illegal PstI site found at 1216
    Illegal PstI site found at 5762
    Illegal PstI site found at 6537
    Illegal PstI site found at 6945
    Illegal PstI site found at 7919
    Illegal PstI site found at 10025
    Illegal PstI site found at 10558
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1176
    Illegal XbaI site found at 44
    Illegal XbaI site found at 2568
    Illegal XbaI site found at 3524
    Illegal XbaI site found at 4276
    Illegal XbaI site found at 5350
    Illegal XbaI site found at 6446
    Illegal XbaI site found at 7369
    Illegal PstI site found at 1216
    Illegal PstI site found at 5762
    Illegal PstI site found at 6537
    Illegal PstI site found at 6945
    Illegal PstI site found at 7919
    Illegal PstI site found at 10025
    Illegal PstI site found at 10558
    Illegal NgoMIV site found at 5585
    Illegal NgoMIV site found at 6025
    Illegal NgoMIV site found at 6792
    Illegal NgoMIV site found at 7927
    Illegal AgeI site found at 3417
    Illegal AgeI site found at 5830
    Illegal AgeI site found at 6989
    Illegal AgeI site found at 9705
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 10079
    Illegal BsaI.rc site found at 10570
    Illegal SapI site found at 10730
    Illegal SapI.rc site found at 180
    Illegal SapI.rc site found at 4038


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