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Part:BBa_M36998

Designed by: Nick Davis, Rachel RoseFigura   Group: Stanford BIOE44 - S11   (2011-04-28)

Coenzyme B-12 Riboswitch for E. coli (nucleotides 1-240)

This part contains the full string of nucleotides for the coenzyme B-12 riboswitch present in Escherichia coli. The aptamer domain of the riboswitch (nucleotides 1-202) undergoes substantial allosteric changes upon binding coenzyme B-12 (aka hydroxocobalamin). The coenzyme B-12 aptamer has one of the most complex metabolite-binding structures of known naturally-occurring riboswitches, though other riboswitches with simpler aptamers can also sense their targets with impressive precision and use resultant structural changes to control gene expression.

Nucleotides 203-240 represent the 5'UTR (untranslated region) upstream of the btuB coding sequence in the E. coli genome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 156
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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