Coding

Part:BBa_M36996

Designed by: Nathan Barnett, Meghan Bowler, Kian Torabian   Group: Stanford BIOE44 - S11   (2011-04-28)


Non-Glycolated Antifreeze Protein (AFP) - 10-Repeated Segment

The standard non-glycolated antifreeze protein (AFP) from the Pseudopleuronecta americanus. Begins with a chain of C's and is followed by 10 rough repeats of "ANAAAAAALTA". The codons have been optimized for E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 32
    Illegal PstI site found at 98
    Illegal PstI site found at 170
    Illegal PstI site found at 182
    Illegal PstI site found at 281
    Illegal PstI site found at 329
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 32
    Illegal PstI site found at 98
    Illegal PstI site found at 170
    Illegal PstI site found at 182
    Illegal PstI site found at 281
    Illegal PstI site found at 329
    Illegal NotI site found at 133
    Illegal NotI site found at 232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 360
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 32
    Illegal PstI site found at 98
    Illegal PstI site found at 170
    Illegal PstI site found at 182
    Illegal PstI site found at 281
    Illegal PstI site found at 329
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 32
    Illegal PstI site found at 98
    Illegal PstI site found at 170
    Illegal PstI site found at 182
    Illegal PstI site found at 281
    Illegal PstI site found at 329
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/antifreeze
Parameters
proteinAntifreeze Protein