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Part:BBa_M36995

Designed by: Nathan Barnett, Meghan Bowler, Kian Torabian   Group: Stanford BIOE44 - S11   (2011-04-28)


Type II Secreted Antifreeze Actuator in E. coli (5-Repeat)

5'UTR, signal peptide, non-glycolated antifreeze protein, and terminator that will as whole transcribe and secrete antifreeze protein outside the cell membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 109
    Illegal PstI site found at 164
    Illegal PstI site found at 191
    Illegal PstI site found at 209
    Illegal PstI site found at 221
    Illegal PstI site found at 257
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 109
    Illegal PstI site found at 164
    Illegal PstI site found at 191
    Illegal PstI site found at 209
    Illegal PstI site found at 221
    Illegal PstI site found at 257
    Illegal NotI site found at 292
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 321
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 109
    Illegal PstI site found at 164
    Illegal PstI site found at 191
    Illegal PstI site found at 209
    Illegal PstI site found at 221
    Illegal PstI site found at 257
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 109
    Illegal PstI site found at 164
    Illegal PstI site found at 191
    Illegal PstI site found at 209
    Illegal PstI site found at 221
    Illegal PstI site found at 257
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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