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Part:BBa_M36994

Designed by: Nathan Barnett, Meghan Bowler, Kian Torabian   Group: Stanford BIOE44 - S11   (2011-04-28)


Type II Secreted Antifreeze Actuator in E. coli (1-Repeat)

5'UTR, signal peptide, non-glycolated antifreeze protein, and terminator that will as whole transcribe and secrete antifreeze protein outside the cell membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 109
    Illegal PstI site found at 164
    Illegal PstI site found at 191
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 109
    Illegal PstI site found at 164
    Illegal PstI site found at 191
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 222
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 109
    Illegal PstI site found at 164
    Illegal PstI site found at 191
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 109
    Illegal PstI site found at 164
    Illegal PstI site found at 191
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
None