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Part:BBa_M36993

Designed by: Nathan Barnett, Meghan Bowler, Kian Torabian   Group: Stanford BIOE44 - S11   (2011-04-28)


Type II Secreted Antifreeze Actuator in E. coli (10-Repeat)

5'UTR, signal peptide, non-glycolated antifreeze protein, and terminator that will as whole transcribe and secrete antifreeze protein outside the cell membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 109
    Illegal PstI site found at 161
    Illegal PstI site found at 224
    Illegal PstI site found at 230
    Illegal PstI site found at 242
    Illegal PstI site found at 254
    Illegal PstI site found at 260
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 109
    Illegal PstI site found at 161
    Illegal PstI site found at 224
    Illegal PstI site found at 230
    Illegal PstI site found at 242
    Illegal PstI site found at 254
    Illegal PstI site found at 260
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 486
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 109
    Illegal PstI site found at 161
    Illegal PstI site found at 224
    Illegal PstI site found at 230
    Illegal PstI site found at 242
    Illegal PstI site found at 254
    Illegal PstI site found at 260
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 109
    Illegal PstI site found at 161
    Illegal PstI site found at 224
    Illegal PstI site found at 230
    Illegal PstI site found at 242
    Illegal PstI site found at 254
    Illegal PstI site found at 260
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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