Composite

Part:BBa_M36992

Designed by: Meghan Bowler, Nathan Barnett, Kian Torabian   Group: Stanford BIOE44 - S11   (2011-05-03)


Type II Secreted Antifreeze Actuator in E.coli (10-Repeat)

5'UTR, signal peptide, non-glycolated antifreeze protein, and terminator that will as whole transcribe and secrete antifreeze protein outside the cell membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 101
    Illegal PstI site found at 145
    Illegal PstI site found at 211
    Illegal PstI site found at 283
    Illegal PstI site found at 295
    Illegal PstI site found at 394
    Illegal PstI site found at 442
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 101
    Illegal PstI site found at 145
    Illegal PstI site found at 211
    Illegal PstI site found at 283
    Illegal PstI site found at 295
    Illegal PstI site found at 394
    Illegal PstI site found at 442
    Illegal NotI site found at 246
    Illegal NotI site found at 345
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 473
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 101
    Illegal PstI site found at 145
    Illegal PstI site found at 211
    Illegal PstI site found at 283
    Illegal PstI site found at 295
    Illegal PstI site found at 394
    Illegal PstI site found at 442
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 101
    Illegal PstI site found at 145
    Illegal PstI site found at 211
    Illegal PstI site found at 283
    Illegal PstI site found at 295
    Illegal PstI site found at 394
    Illegal PstI site found at 442
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None