Composite

Part:BBa_M36991

Designed by: Nathan Barnett, Meghan Bowler, Kian Torabian   Group: Stanford BIOE44 - S11   (2011-05-03)


Type II Secreted Antifreeze Actuator in E. coli (1-Repeat)

5'UTR, signal peptide, non-glycolated antifreeze protein, and terminator that will as whole transcribe and secrete antifreeze protein outside the cell membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 101
    Illegal PstI site found at 148
    Illegal PstI site found at 175
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 101
    Illegal PstI site found at 148
    Illegal PstI site found at 175
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 206
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 101
    Illegal PstI site found at 148
    Illegal PstI site found at 175
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 101
    Illegal PstI site found at 148
    Illegal PstI site found at 175
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None