Composite

Part:BBa_M36990

Designed by: Nathan Barnett, Meghan Bowler, Kian Torabian   Group: Stanford BIOE44 - S11   (2011-05-03)


Type II Secreted Antifreeze Actuator in E. coli (5-Repeat)

5'UTR, signal peptide, non-glycolated antifreeze protein, and terminator that will as whole transcribe and secrete antifreeze protein outside the cell membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 101
    Illegal PstI site found at 148
    Illegal PstI site found at 175
    Illegal PstI site found at 193
    Illegal PstI site found at 205
    Illegal PstI site found at 241
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 101
    Illegal PstI site found at 148
    Illegal PstI site found at 175
    Illegal PstI site found at 193
    Illegal PstI site found at 205
    Illegal PstI site found at 241
    Illegal NotI site found at 276
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 305
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 101
    Illegal PstI site found at 148
    Illegal PstI site found at 175
    Illegal PstI site found at 193
    Illegal PstI site found at 205
    Illegal PstI site found at 241
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 101
    Illegal PstI site found at 148
    Illegal PstI site found at 175
    Illegal PstI site found at 193
    Illegal PstI site found at 205
    Illegal PstI site found at 241
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None