Composite

Part:BBa_M36983

Designed by: Nathan Barnett, Meghan Bowler, Kian Torabian   Group: Stanford BIOE44 - S11   (2011-05-17)


Type II Secreted Antifreeze Actuator in E. coli (5-Repeat) Correct

5'UTR, signal peptide, non-glycolated antifreeze protein, and terminator that will as whole transcribe and secrete antifreeze protein outside the cell membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 107
    Illegal PstI site found at 154
    Illegal PstI site found at 181
    Illegal PstI site found at 199
    Illegal PstI site found at 211
    Illegal PstI site found at 247
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 107
    Illegal PstI site found at 154
    Illegal PstI site found at 181
    Illegal PstI site found at 199
    Illegal PstI site found at 211
    Illegal PstI site found at 247
    Illegal NotI site found at 282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 311
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 107
    Illegal PstI site found at 154
    Illegal PstI site found at 181
    Illegal PstI site found at 199
    Illegal PstI site found at 211
    Illegal PstI site found at 247
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 107
    Illegal PstI site found at 154
    Illegal PstI site found at 181
    Illegal PstI site found at 199
    Illegal PstI site found at 211
    Illegal PstI site found at 247
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None