Composite
Part:BBa_M36982
Designed by: Nathan Barnett, Meghan Bowler, Kian Torabian Group: Stanford BIOE44 - S11 (2011-05-17)
Type II Secreted Antifreeze Actuator in E. coli (1-Repeat) Correct
5'UTR, signal peptide, non-glycolated antifreeze protein, and terminator that will as whole transcribe and secrete antifreeze protein outside the cell membrane.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 107
Illegal PstI site found at 154
Illegal PstI site found at 181 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 107
Illegal PstI site found at 154
Illegal PstI site found at 181 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 212
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 107
Illegal PstI site found at 154
Illegal PstI site found at 181 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 107
Illegal PstI site found at 154
Illegal PstI site found at 181 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |