Composite

Part:BBa_M36979

Designed by: Nick Davis, Rachel RoseFigura   Group: Stanford BIOE44 - S11   (2011-05-03)

Coenzyme B-12 Biosensor for E. coli (

Composite part—a Vitamin B12 Biosensor designed specifically for use in Escherichia coli (in whose genome the vitamin B12 riboswitch is naturally present). This part is composed of: 1) a strong, constitutive promoter (J23100; ~99% promotion efficiency); 2) the Coenzyme B12 Riboswitch (M36998)—portion of RNA that forms complex aptamer structure upon binding of coenzyme B12 as substrate; 3) Gemini fusion protein coding sequence (E0051); 4) a strong terminator (M36010; ~99% termination efficiency).

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 191
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1153


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Categories
Parameters
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