Composite

Part:BBa_M36953

Designed by: Amiel Stephen Paz, William Kindschuh, Vickram Gidwani   Group: Stanford BIOE44 - S11   (2015-10-24)


Insect antifreeze protein (RiAFP) actuator

Codes for Rhagium inquisitor antifreeze protein structure identified by Hakim et al. This sequence consists of two protein chains forming a crystal structure with inward-facing ice-binding surfaces. CDS for protein chains separated by a T2A self-cleaving peptide sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 220
    Illegal PstI site found at 517
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 602
    Illegal SpeI site found at 220
    Illegal PstI site found at 517
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 220
    Illegal PstI site found at 517
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 220
    Illegal PstI site found at 517
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None