Regulatory

Part:BBa_M36844

Designed by: Jasmine Johnson, Katie Plummer, Matthew Ryan Frankel   Group: Stanford BIOE44 - S11   (2015-10-25)

p14ARF enhancer and promoter

This part includes the p14ARF enhancer and promoter regions. Many oncogenes, including E2F1 and myc, are attracted to the p14ARF enhancer and bind to the promoter, initiating transcription of the p14aARF gene. p14ARF is an alternate reading frame protein that can activate p53, inducing apoptosis. This sensor allows for the detection of p14ARF when inserted into a S. cerevisiae sensor test rig (e.g. part BBa_M36093) or a mammalian sensor test rig (e.g. part BBa_M36097), that contains a GFP coding sequence. Thus, cells transcribing the p14ARF enhancer and promoter sequence will be detectable through a Fluorescent Report Assay.

Stanford Location: BIOE 44 Fall 2015 Box Barcodes 0133027026 (Mammalian optimized), 0133021781 (Mammalian optimized) and 0133027011 (S. cerevisiae optimized).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 474
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 474
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 474
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 474
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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