Coding

Part:BBa_M36843

Designed by: Jasmine, Katie, Matthew (Bluegreen)   Group: Stanford BIOE44 - S11   (2015-10-24)

E2F1 protein coding sequence

This sequence codes for the transcription factor, E2F1. E2F1 is a member of the E2 family and plays a role in the cell cycle and activation of tumor suppressor proteins. It has been shown to be involved in processes related to transcriptional control, apoptosis and dna damage control through its particpation in p53 dependent pathways. In particular, E2F1 directly drives the expression of p14ARF, a tumor supressor gene that upregulates p53.

E2F1 can be used by encoding its DNA sequence(provided here) to transcriptionally activate certain genes involved in apoptosis (i.e. p14ARF) as well as the cell cycle(i.e. CDC2).

Stanford Location: Glycerol Stock, BIOE 44 Fall 2015 box barcode #0133027010

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1242
    Illegal PstI site found at 437
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 437
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 183
    Illegal BglII site found at 754
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1242
    Illegal PstI site found at 437
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1242
    Illegal PstI site found at 437
    Illegal AgeI site found at 145
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None