Composite

Part:BBa_M36827

Designed by: Leah Chase   Group: Stanford BIOE44 - S11   (2014-10-22)


Zinc Sensor

This composite part contains a promoter and ribosome-binding-site, a gene for ZntR, a terminator, and a promoter for ZntA. In the absence of Zinc, the ZntR protein binds to the ZntA binding site. In the presence of zinc, ZntR undergoes a conformation, does not attach to the binding site, and allows for transcription of ZntA. Essentially, this part acts as a sensor for the presence of zinc.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 475
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 364
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 475
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 475
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None