Composite

Part:BBa_M36826

Designed by: Christine Chang, Sonia Targ   Group: Stanford BIOE44 - S11   (2014-10-24)

Phenylalanine Hydroxylase w/ RBS and Terminator

This part codes for the phenylalanine hydroxylase protein. Phenylalanine hydroxylase is an enzyme that converts phenylalanine substrate into tyrosine. This specific part includes a strong RBS from DNA 2.0 and a strong terminator pT-T7 from bacteriophage.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1160
    Illegal PstI site found at 719
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1160
    Illegal NheI site found at 1386
    Illegal PstI site found at 719
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1160
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1160
    Illegal PstI site found at 719
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1160
    Illegal PstI site found at 719
    Illegal NgoMIV site found at 283
    Illegal NgoMIV site found at 1052
    Illegal AgeI site found at 731
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None