Part:BBa_M36782

PoPS -> Melamine deaminase

This actuator's purpose is to code for the output of the enzyme melamine deaminase. This enzyme eliminates an amine group from melamine to produce ammonia and ammeline and is able to remove another amine group at a rate 10 times slower than the first reaction to bring the compound to ammelide. The enzyme does not react to a measurable extent with this last compound, so it metabolizes melamine by consecutive and predictable deaminations. Its design comprises three components, which include a bicistronic ribosome binding site, the MDA gene of interest which codes from melamine deaminase, and a transcription terminator. The part needs a PoPS signal as an input in order to produce the desired enzyme and was designed to work in E. coli.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 899
  Illegal PstI site found at 1463
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 899
  Illegal PstI site found at 1463
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 899
  Illegal PstI site found at 1463
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 899
  Illegal PstI site found at 1463
  Illegal NgoMIV site found at 220
  Illegal NgoMIV site found at 856
  
• 1000
  COMPATIBLE WITH RFC[1000]