Device

Part:BBa_M36763

Designed by: Andrew Lee   Group: Stanford BIOE44 - S11   (2014-10-23)


Yeast-Optimized Chicken Lysozyme (LysA) Secretor

Our device can be transformed into yeast for the production of mammalian lysozyme. It consists of a GAL1 promoter, the LysA gene linked to a yeast-optimized albumin signal peptide, and a Cyc1 terminator. Chicken lysozyme (LysA) has been shown to have high catalytic activity against gram-positive bacteria, hydrolyzing the peptidoglycan in the bacterial cell wall. The albumin signal peptide is optimized for lytic enzyme secretion in yeast expression hosts. By granting ethanologens, like yeast, the ability to secrete lysozyme, this part can be used to simultaneously decontaminate broths while carrying out fermentation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 983
    Illegal NgoMIV site found at 1026
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None