Coding
MDA

Part:BBa_M36761

Designed by: Ray Paul Laureano-Mercado & Mark Kirollos   Group: Stanford BIOE44 - S11   (2013-06-10)


Melamine deaminase coding sequence

This part is the coding sequence for the enzyme melamine deaminase, optimized for usage in E. coli. This enzyme eliminates an amine group from melamine to produce ammonia and ammeline and is able to remove another amine group at a rate 10 times slower than the first reaction to bring the compound to ammelide. The enzyme does not react to a measurable extent with this last compound, so it metabolizes melamine by consecutive and predictable deaminations.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 814
    Illegal PstI site found at 1378
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 814
    Illegal PstI site found at 1378
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 814
    Illegal PstI site found at 1378
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 814
    Illegal PstI site found at 1378
    Illegal NgoMIV site found at 135
    Illegal NgoMIV site found at 771
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//direction/forward
//dna
Parameters
proteinmelamine deaminase