Coding

Part:BBa_M36705

Designed by: Paul Chen, Sean Valle, Svetlana Lyalina   Group: Stanford BIOE44 - S11   (2011-04-26)

Benzalacetone Reductase

Coding sequence for the enzyme benzalacetone reductase. This enzyme reduces 4-hydroxybenzalacetone to 4-(4-Hydroxyphenyl)butan-2-one (raspberry ketone), the primary contributor to raspberry flavor and smell.

No mutations were made to the enzymes genetic sequence. The genetic sequence is optimized for use in Escherichia Coli.

Usage and Biology

BE WARY OF USING THIS PART. SEE EXPERIENCE PAGE FOR DETAILS, AS THIS ENZYME HAS BEEN SHOWN TWICE SEPARATELY NOT TO REDUCE BENZALACETONE. Cambridge-JIC 2014.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 759
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 267
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 715
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 759
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 759
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 112


[edit]
Categories
//cds/enzyme
Parameters
ec_num1.3.1.-