Part:BBa_M36667

FNR-dependent Hypoxia Sensor

This part utilizes fumarate nitrate reductase (FNR) transcription factor with E. Coli constitutive promoter, which regulates pepT7 promoter downstream with Gemini actuator. FNR and pepT7 promoter sequence are from Salmonella typhimurium, and the constitutive promoter, ribosome binding site, and terminator are generic E. Coli parts.

FNR is regulated by O2: inactive when exposed to O2, and requires low O2 (hypoxia) and glutathione/cysteine desulfurase to reactivate, of which cysteine desulfurase is already present within E. Coli. Once activated, FNR induces the pepT7 promoter, which facilitates expression of the Gemini actuator. The lacZ portion of Gemini will produce beta-galactosidase, which can be qualitatively and quantitatively measured.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 610
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 610
  Illegal NheI site found at 2
  Illegal NheI site found at 25
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 610
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 610
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 610
  
• 1000
  COMPATIBLE WITH RFC[1000]