Composite

Part:BBa_M36666

Designed by: Ian Hull, Poorwa Godbole   Group: Stanford BIOE44 - S11   (2014-04-30)


HeLa beta-galactosidase actuator with secretion tag

This actuator is designed to produce beta-galactosidase with an N-terminal secretion tag in HeLa cells. It links the alpha and omega fragments of the E. coli lacZ gene, though the codons have been optimized for expression in HeLa cells. It includes the kozak sequence immediately before the start codon and a secretion tag immediately after the start codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 236
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 411
    Illegal BsaI site found at 1025
    Illegal BsaI.rc site found at 934


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Categories
Parameters
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