Coding

Part:BBa_M36660

Designed by: Taylor Marie Chavez, Maria Iglesias, Alison Jahansouz   Group: Stanford BIOE44 - S11   (2015-10-23)

mRNA Sequence for Poly-beta-Hydroxybutyrate Polymerase

Part codes for the mRNA sequence to produce poly-beta-hydroxybutyrate polymerase, which synthesizes poly-3-hydroxybutyrate in from (R)-3-hydroxybutyl-CoA. This composite part is simply the DNA sequence collected from an amino acid sequence obtained from Xanthomonas campestris pv. raphani 756C, specifically from the gene G0CFI5. Part is intended to be inserted into the bacterial expression vector pD431-SR from DNA 2.0, with a low copy number and kanamycin resistance. Complete system should be transformed into E. Coli.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 250
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 250
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 246
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 250
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None