Part:BBa_M36604
Heat-->PoPS (with postive feedback loop)
This composite part is a sensor that focuses on creating a positive feedback loop to increase the PoPS signal for a desired actuator further downstream. In this design, Sigma-32, a sigma factor turned on when E. coli experiences heat shock, is the transcription factor for the htpG Heat Shock Promoter (BBa_J45504). Once active, the inducible promoter allows for, with the assistance of a medium strength bicistronic RBS (BBa_M36405), the transcription of rpoH (BBa_M36888), the sequence for sigma-32. Such a design creates a positive feedback loop, creating more sigma-32 from a small initial heat exposure. This is solely done in order to generate a larger PoPS signal, which would allow for a greater production of one's desired downstream production.
Device input is heat exposure. Device output is a PoPS signal.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1108
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1108
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1108
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1108
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 810
Illegal SapI.rc site found at 1159
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