Device

Part:BBa_M36598

Designed by: Daniel Hu, Andrew Austin Lee, Clinton Olivas   Group: Stanford BIOE44 - S11   (2014-10-24)

Yeast-Optimized Chicken Lysozyme (LysC) Secretor

Our device can be transformed into yeast for the production of mammalian lysozyme. It consists of a GAL1 promoter, the LysC gene linked to a yeast-optimized albumin signal peptide, and a Cyc1 terminator. Chicken lysozyme (LysC) has been shown to have high catalytic activity against gram-positive bacteria, hydrolyzing the peptidoglycan in the bacterial cell wall. The albumin signal peptide is optimized for lytic enzyme secretion in yeast expression hosts. By granting ethanologens, like yeast, the ability to secrete lysozyme, this part can be used to simultaneously decontaminate broths while carrying out fermentation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 770
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 989
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None