Coding

Part:BBa_M36582

Designed by: Aris Kare   Group: Stanford BIOE44 - S11   (2015-10-24)

ctfB Gene Sequence

This part is the ctfB gene, or butyrate-acetoacetate COA-transferase subunit B, that is used as part of a metabolic pathway in Clostridium acetobutylicum ATCC 824 bacteria to produce acetone. We are aiming to use this gene, along with several others, to eventually produce acetone and CO2 in E.coli bacteria. This part, along with ctfA, produces acetoacetate, acetyl-CoA, and butyryl-CoA, which are the penultimate products of the C. acetobutylicum acetone pathway before the final product, acetone.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 42
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 42
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 42
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/actfB Gene Sequence